Publications

A graphical abstract of the publication Covalent inhibition of the SARS-CoV-2 NiRAN domain via an active-site cysteine

Nov. 25, 2024

Covalent inhibition of the SARS-CoV-2 NiRAN domain via an active-site cysteine

Genaro Hernandez, Adam Osinski, Abir Majumdar, Jennifer L Eitson, Monika Antczak, Krzysztof Pawlowski, Hanspeter Niederstrasser, Kelly A Servage, Bruce Posner, John W Schoggins, Joseph A. Ready & Vincent S Tagliabracci

Description
We report a covalent small-molecule inhibitor of the SARS-CoV-2 NiRAN domain derived from a high-throughput screen.
A graphical abstract of the publication Biochemical and structural insights into a 5’ to 3’ RNA ligase reveal a potential role in tRNA ligation

Oct. 10, 2024

Biochemical and structural insights into a 5’ to 3’ RNA ligase reveal a potential role in tRNA ligation

Yingjie Hu, Victor A. Lopez, Hengyi Xu, James P. Pfister, Bing Song, Kelly A. Servage, Masahiro Sakurai, Benjamin T. Jones, Joshua T. Mendell, Tao Wang, Jun Wu, Alan M. Lambowitz, Diana R. Tomchick, Krzysztof Pawłowski & Vincent S. Tagliabracci

Description
We show that an atypical ATP-grasp enzyme, C12orf29, is a 5' to 3' RNA ligase that can repair tRNAs.
A graphical abstract of the publication A domain of all trades: The enzymatic versatility of the NiRAN domain

Nov. 2, 2023

A domain of all trades: The enzymatic versatility of the NiRAN domain

Adam Osinski & Vincent S. Tagliabracci

Description
A short preview of a publication that, through a series of cryo-EM structures, demonstrates the versatility of the SARS-CoV-2 NiRAN domain.
A graphical abstract of the publication The mechanism of RNA capping by SARS-CoV-2

Aug. 9, 2022

The mechanism of RNA capping by SARS-CoV-2

Gina J. Park*, Adam Osinski*, Genaro Hernandez, Jennifer L. Eitson, Abir Majumdar, Marco Tonelli, Katie Henzler-Wildman, Krzysztof Pawłowski, Zhe Chen, Yang Li, John W. Schoggins & Vincent S. Tagliabracci

Description
We discovered that "RNAylation" of a protein substrate by a SARS-CoV-2 pseudokinase is a critical step in the virus' quest to cap and thus protect its genome.
A graphical abstract of the publication Methods for discovering catalytic activities for pseudokinases

May 4, 2022

Methods for discovering catalytic activities for pseudokinases

Miles H. Black, Marcin Gradowski, Krzysztof Pawłowski & Vincent S. Tagliabracci

Description
We lay out our strategy for discovering what mysterious pseudokinases do.
A graphical abstract of the publication Structural and mechanistic basis for protein glutamylation by the kinase fold

Nov. 4, 2021

Structural and mechanistic basis for protein glutamylation by the kinase fold

Adam Osinski, Miles H. Black, Krzysztof Pawłowski, Zhe Chen, Yang Li & Vincent S. Tagliabracci

Description
We solved a cryo-EM structure for a legionella effector complex to gain insight into the mechanism by which it functions.
A graphical abstract of the publication Dynamic remodeling of host membranes by self-organizing bacterial effectors

May 28, 2021

Dynamic remodeling of host membranes by self-organizing bacterial effectors

Ting-Sung Hsieh*, Victor A. Lopez*, Miles H. Black*, Adam Osinski, Krzysztof Pawłowski, Diana R. Tomchick, Jen Liou & Vincent S. Tagliabracci

Description
We found a self-organizing system consisting of a bacterial phosphoinositide kinase (MavQ) and its opposing phosphatase (SidP) that formed spatiotemporal patterns, including traveling waves, to remodel host cellular membranes.
A graphical abstract of the publication A Legionella effector ADP-ribosyltransferase inactivates glutamate dehydrogenase

Jan. 18, 2021

A Legionella effector ADP-ribosyltransferase inactivates glutamate dehydrogenase

Miles H. Black, Adam Osinski, Gina J. Park, Marcin Gradowski, Kelly A. Servage, Krzysztof Pawłowski & Vincent S. Tagliabracci

Description
We found that a legionella effector, Lart1, targets a conserved arginine on, and thereby inactivates, a set of host glutamate dehydrogenase enzymes.
A graphical abstract of the publication A Legionella effector kinase is activated by host inositol hexakisphosphate

March 20, 2020

A Legionella effector kinase is activated by host inositol hexakisphosphate

Anju Sreelatha, Christine Nolan , Brenden C. Park, Krzysztof Pawłowski, Diana R. Tomchick & Vincent S. Tagliabracci

Description
We found that the legionella effector lpg2603 is a kinase that requires inositol hexakisphosphate for activity.
A graphical abstract of the publication A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity

Sept. 12, 2019

A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity

Victor A. Lopez*, Brenden C. Park*, Dominika Nowak, Anju Sreelatha, Patrycja Zembek, Jessie Fernandez, Kelly A. Servage, Marcin Gradowski, Jacek Hennig, Diana R. Tomchick, Krzysztof Pawłowski, Magdalena Krzymowska & Vincent S. Tagliabracci

Description
We discovered that the bacterial effector HopBF1 has a kinase fold, and that the protein exploits this fold to inactivate Hsp90 in eukaryotic hosts.
A graphical abstract of the publication Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases

May 24, 2019

Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases

Miles H. Black, Adam Osinski, Marcin Gradowski, Kelly A Servage, Krzysztof Pawłowski, Diana R. Tomchick & Vincent S. Tagliabracci

Description
We show that the legionella metaeffector SidJ, which has a kinase fold, polyglutamylates and thereby inactivates its substrate SidE.
A graphical abstract of the publication Thinking outside of the cell: Secreted protein kinases in bacteria, parasites, and mammals

April 2, 2019

Thinking outside of the cell: Secreted protein kinases in bacteria, parasites, and mammals

Brenden C. Park, Michael Reese & Vincent S. Tagliabracci

Description
A review of secreted kinases.
A graphical abstract of the publication Protein AMPylation by an Evolutionarily Conserved Pseudokinase

Oct. 18, 2018

Protein AMPylation by an Evolutionarily Conserved Pseudokinase

Anju Sreelatha, Samantha S. Yee, Victor A. Lopez, Brenden C. Park, Lisa N. Kinch, Sylwia Pilch, Kelly A. Servage, Junmei Zhang, Jenny Jiou, Monika Karasiewicz-Urbańska, Małgorzata Łobocka, Nick V. Grishin, Kim Orth, Roza Kucharczyk, Krzysztof Pawłowski, Diana R. Tomchick & Vincent S. Tagliabracci

Description
We found that the conserved protein SelO adopts a kinase domain, but binds ATP backwards. Upon further investigation, we determined that SelO catalyzes the transfer of the AMP from the nucleotide to protein substrates, a function that is important in cellular redox homeostasis.
A graphical abstract of the publication Phosphorylation of spore coat proteins by a family of atypical protein kinases

June 21, 2016

Phosphorylation of spore coat proteins by a family of atypical protein kinases

Kim B. Nguyen*, Anju Sreelatha*, Eric S. Durrant, Javier Lopez-Garrido, Anna Muszewska, Małgorzata Dudkiewicz, Marcin Grynberg, Samantha Yee, Kit Pogliano, Diana R. Tomchick, Krzysztof Pawłowski, Jack E. Dixon & Vincent S. Tagliabracci

Description
We discovered that the bacterial protein CotH is a protein kinase, activity of which is required for spore germination. We further determined that the substrates of CotH are two genomic neighbors, CotB and CotG.
A graphical abstract of the publication The secretory pathway kinases

Sept. 16, 2015

The secretory pathway kinases

Anju Sreelatha, Lisa N. Kinch & Vincent S. Tagliabracci

Description
A review of kinases that phosphorylate secreted proteins.
A graphical abstract of the publication A Single Kinase Generates the Majority of the Secreted Phosphoproteome

June 18, 2015

A Single Kinase Generates the Majority of the Secreted Phosphoproteome

Vincent S. Tagliabracci*, Sandra E. Wiley*, Xiao Guo, Lisa N. Kinch, Eric Durrant, Jianzhong Wen, Junyu Xiao, Jixin Cui, Kim B. Nguyen, James L. Engel, Joshua J. Coon, Nick Grishin, Lorenzo A. Pinna, David J. Pagliarini & Jack E. Dixon

Description
We demonstrate that the kinase Fam20C phosphorylates the majority of extracellular phosphoproteins.
A graphical abstract of the publication Dynamic regulation of FGF23 by Fam20C phosphorylation, GalNAc-T3 glycosylation, and furin proteolysis

March 26, 2014

Dynamic regulation of FGF23 by Fam20C phosphorylation, GalNAc-T3 glycosylation, and furin proteolysis

Vincent S. Tagliabracci, James L. Engel, Sandra E. Wiley, Junyu Xiao, David J. Gonzalez, Hitesh Nidumanda Appaiah, Antonius Koller, Victor Nizet, Kenneth E. White & Jack E. Dixon

Description
We show that the regulation of FGF23 is mediated through its phosphorylation by Fam20C.
A graphical abstract of the publication Secreted protein kinases

Dec. 29, 2012

Secreted protein kinases

Vincent S. Tagliabracci, Lorenzo A. Pinna & Jack E. Dixon

Description
A review of secreted protein kinases.
A graphical abstract of the publication Secreted Kinase Phosphorylates Extracellular Proteins That Regulate Biomineralization

May 10, 2012

Secreted Kinase Phosphorylates Extracellular Proteins That Regulate Biomineralization

Vincent S. Tagliabracci, James L. Engel, Jianzhong Wen, Sandra E. Wiley, Carolyn A. Worby, Lisa N. Kinch, Junyu Xiao, Nick V. Grishin & Jack E. Dixon

Description
We identified Fam20C as the bona fide casein kinase.
A graphical abstract of the publication Phosphate incorporation during glycogen synthesis and Lafora disease

March 2, 2011

Phosphate incorporation during glycogen synthesis and Lafora disease

Vincent S. Tagliabracci, Christian Heiss, Chandra Karthik, Christopher J. Contreras, John Glushka, Mayumi Ishihara, Parastoo Azadi, Thomas D. Hurley, Anna A. DePaoli-Roach & Peter J. Roach

Description
We demonstrate that glycogen synthase can introduce phosphates into glycogen, we propose a mechanism by which it works, and we show that the phosphatase laforin can remove these phosphates from glycogen.
A graphical abstract of the publication Abnormal metabolism of glycogen phosphate as a cause for Lafora disease

Dec. 5, 2008

Abnormal metabolism of glycogen phosphate as a cause for Lafora disease

Vincent S. Tagliabracci, Jean Marie Girard, Dyann Segvich, Catalina Meyer, Julie Turnbull, Xiaochu Zhao, Berge A. Minassian, Anna A. Depaoli-Roach & Peter J. Roach

Description
We show that glycogen in middle-aged mice lacking the phosphatase laforin is defective due to the overaccumulation of phosphates.
A graphical abstract of the publication Laforin is a glycogen phosphatase, deficiency of which leads to elevated phosphorylation of glycogen in vivo

Dec. 4, 2007

Laforin is a glycogen phosphatase, deficiency of which leads to elevated phosphorylation of glycogen in vivo

Vincent S. Tagliabracci, Julie Turnbull, Wei Wang, Jean-Marie Girard, Xiaochu Zhao, Alexander V. Skurat, Antonio V. Delgado-Escueta, Berge A. Minassian, Anna A. DePaoli-Roach & Peter J. Roach

Description
We show that glycogen is a substrate for the phosphatase laforin. We quantify the rates and extent of glycogen dephosphorylation by laforin in vitro.

Tagliabracci lab
6000 Harry Hines Blvd
NA5.214
Dallas, TX 75390